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ANTIGEN TESTS

In an antigen test, scientists used specially designed antibodies to look for small pieces of the virus present in a patient sample. These pieces of the virus are the antigens. Because antibodies only recognize one antigen, if the antibody sticks onto something we know it must be a piece of the virus, telling us that Coronavirus is present in the sample.

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TESTING PROCESS

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Sergii Iaremenko/Science Photo Library/Getty Images

The antigen testing process is very similar to how a home pregnancy test operates. A sample from a patient is placed in a small well, which we can see in the photo on the right is labeled with an 'S'. The liquid sample then moves up the strip, just as water moves up a paper napkin. The 'C' stands for control. That band appears to let us know that the sample was drawn up, and that the test is functioning successfully. The 'T' is the test band. If this band appears, we know that the sample contains Coronavirus. So how does this band work?

 

In creating the test, special antibodies that detect Coronavirus pieces (antigens) are adhered to the strip. They are placed in a horizontal band, and they are part of what helps to form the visual bands we see. Another antibody is also added that are designed to attach to the same antigens, but on the opposite side. These are placed onto the strip, but not adhered. As the sample flows up the strip, it will run into these free antibodies, and if there is a virus antigen in the sample, these antibodies will adhere to it, and continue to flow upward with the sample. These antibodies also have a marker on them that activate once they interact with one of the adhered antibodies. The image on the right helps to visualize what the positive test band looks like up close. For a better understanding of how the process works in motion, check out the video below.

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“Rapid Antigen Test.” Wikipedia, en.wikipedia.org/wiki/Rapid_antigen_test.

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Racaniello, Vincent. “Detection of Antigens or Antibodies by ELISA.” Virology Blog, www.virology.ws/2010/07/16/detection-of-antigens-or-antibodies-by-elisa/.

GeneTexAntibody, director. How a Rapid Virus Antigen Test Works. YouTube, YouTube, 10 May 2020, www.youtube.com/watch?v=PkczTPfIE20.

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